Δ9-Tetrahydrocannabinol induces the apoptotic pathway in cultured cortical neurones via activation of the CB1 receptor

E. Downer, B. Boland, M. Fogarty, V. Campbell

Research output: Contribution to journalArticlepeer-review

77 Citations (Scopus)

Abstract

Δ9-Tetrahydrocannabinol, the principal psychoactive component of marijuana, exerts a variety of effects on the CNS, including impaired cognitive function and neurobehavioural deficits. The mechanisms underlying these neuronal responses to tetrahydrocannabinol are unclear but may involve alterations in neuronal viability. Tetrahydrocannabinol has been shown to influence neuronal survival but the role of the cannabinoid receptors in the regulation of neuronal viability has not been fully clarified. In this study we demonstrate that tetrahydrocannabinol promotes the release of cytochrome c, activates caspase-3, promotes cleavage of the DNA repair enzyme poly-ADP ribose polymerase and induces DNA fragmentation in cultured cortical neurones. These effects of tetrahydrocannabinol were completely abrogated by the CB1 receptor antagonist AM-251. The findings of this study demonstrate that tetrahydrocannabinol induces apoptosis in cortical neurones in a manner involving the CB1 subtype of cannabinoid receptor.

Original languageEnglish
Pages (from-to)3973-3978
Number of pages6
JournalNeuroReport
Volume12
Issue number18
DOIs
Publication statusPublished - 21 Dec 2001
Externally publishedYes

Keywords

  • Apoptosis
  • Cannabinoid receptor
  • Caspase-3
  • Poly-ADP-ribose polymerase
  • Tetrahydrocannabinol

Fingerprint

Dive into the research topics of 'Δ9-Tetrahydrocannabinol induces the apoptotic pathway in cultured cortical neurones via activation of the CB1 receptor'. Together they form a unique fingerprint.

Cite this