TY - JOUR
T1 - A dry immersion model of microgravity modulates platelet phenotype, miRNA signature, and circulating plasma protein biomarker profile
AU - Twomey, Laura
AU - Navasiolava, Nastassia
AU - Robin, Adrien
AU - Bareille, Marie Pierre
AU - Gauquelin-Koch, Guillemette
AU - Beck, Arnaud
AU - Larcher, Françoise
AU - Meade-Murphy, Gerardene
AU - Sheridan, Sinead
AU - Maguire, Patricia B.
AU - Harrison, Michael
AU - Degryse, Bernard
AU - Moyna, Niall M.
AU - Gharib, Claude
AU - Custaud, Marc Antoine
AU - Murphy, Ronan P.
N1 - Funding Information:
This dry immersion protocol was supported by CNES (DAR CNES N° 2018-4800000970). R.P.M. acknowledges the funding support of Science Foundation Ireland (11/TIDA/B1927), Health Research Board of Ireland (HRA/2009/122), Enterprise Ireland and the European Regional Development Fund (ERDF) under Ireland’s European Structural and Investment Funds programmes (PC-2009-0311, INV/13000/001/2015, IP 2015 0399Y, IP 2015 0418 & IP 2017 0587). L.T. was in part supported by an Irish Research Council for Science, Engineering & Technology (IRCSET) EMBARK Postgraduate Scholarship 2012–2015 (RS/2012/2499). RPM is a management committee member of the EU H2020 COST actions-CM1406 & CA18127.
Publisher Copyright:
© 2021, The Author(s).
PY - 2021/12
Y1 - 2021/12
N2 - Ground based research modalities of microgravity have been proposed as innovative methods to investigate the aetiology of chronic age-related conditions such as cardiovascular disease. Dry Immersion (DI), has been effectively used to interrogate the sequelae of physical inactivity (PI) and microgravity on multiple physiological systems. Herein we look at the causa et effectus of 3-day DI on platelet phenotype, and correlate with both miRomic and circulating biomarker expression. The miRomic profile of platelets is reflective of phenotype, which itself is sensitive and malleable to the exposome, undergoing responsive transitions in order to fulfil platelets role in thrombosis and haemostasis. Heterogeneous platelet subpopulations circulate at any given time, with varying degrees of sensitivity to activation. Employing a DI model, we investigate the effect of acute PI on platelet function in 12 healthy males. 3-day DI resulted in a significant increase in platelet count, plateletcrit, platelet adhesion, aggregation, and a modest elevation of platelet reactivity index (PRI). We identified 15 protein biomarkers and 22 miRNA whose expression levels were altered after DI. A 3-day DI model of microgravity/physical inactivity induced a prothrombotic platelet phenotype with an unique platelet miRNA signature, increased platelet count and plateletcrit. This correlated with a unique circulating protein biomarker signature. Taken together, these findings highlight platelets as sensitive adaptive sentinels and functional biomarkers of epigenetic drift within the cardiovascular compartment.
AB - Ground based research modalities of microgravity have been proposed as innovative methods to investigate the aetiology of chronic age-related conditions such as cardiovascular disease. Dry Immersion (DI), has been effectively used to interrogate the sequelae of physical inactivity (PI) and microgravity on multiple physiological systems. Herein we look at the causa et effectus of 3-day DI on platelet phenotype, and correlate with both miRomic and circulating biomarker expression. The miRomic profile of platelets is reflective of phenotype, which itself is sensitive and malleable to the exposome, undergoing responsive transitions in order to fulfil platelets role in thrombosis and haemostasis. Heterogeneous platelet subpopulations circulate at any given time, with varying degrees of sensitivity to activation. Employing a DI model, we investigate the effect of acute PI on platelet function in 12 healthy males. 3-day DI resulted in a significant increase in platelet count, plateletcrit, platelet adhesion, aggregation, and a modest elevation of platelet reactivity index (PRI). We identified 15 protein biomarkers and 22 miRNA whose expression levels were altered after DI. A 3-day DI model of microgravity/physical inactivity induced a prothrombotic platelet phenotype with an unique platelet miRNA signature, increased platelet count and plateletcrit. This correlated with a unique circulating protein biomarker signature. Taken together, these findings highlight platelets as sensitive adaptive sentinels and functional biomarkers of epigenetic drift within the cardiovascular compartment.
UR - http://www.scopus.com/inward/record.url?scp=85118663155&partnerID=8YFLogxK
U2 - 10.1038/s41598-021-01335-x
DO - 10.1038/s41598-021-01335-x
M3 - Article
C2 - 34753989
AN - SCOPUS:85118663155
VL - 11
JO - Scientific Reports
JF - Scientific Reports
SN - 2045-2322
IS - 1
M1 - 21906
ER -