A gene of the opsin family in the carotenoid gene cluster of Fusarium fujikuroi

Maria M. Prado; Alfonso Prado-Cabrero; Rafael Fernández-Martín; Javier Avalos

doi:10.1007/s00294-004-0508-6

A gene of the opsin family in the carotenoid gene cluster of Fusarium fujikuroi

Maria M. Prado, Alfonso Prado-Cabrero, Rafael Fernández-Martín, Javier Avalos

Research output: Contribution to journal › Article › peer-review

65 Citations (Scopus)

Abstract

Opsins are membrane photoreceptors closely related to the heat-shock proteins of the HSP30 family. Their functions include light-driven ion pumping in archaea and light detection in algae and animals, using the apocarotenoid retinal as a light-absorbing prosthetic group. We describe a gene of Fusarium fujikuroi, carO, coding for a polypeptide resembling opsins and HSP30-like proteins and contiguous to the genes of the carotenoid pathway, carRA and carB. Transcription of carO is induced by light and is deregulated in carotenoid-overproducing mutants. The same regulation pattern is exhibited by carRA and carB; and common conserved DNA elements are found in the three promoters. Heat shock resulted in a modest induction of carO transcription, similar to the one exhibited by carB, confirming a common regulation. Targeted mutagenesis of carO produced no apparent phenotypic modification, including no change in the photoinduction of carotenoid biosynthesis.

Original language	English
Pages (from-to)	47-58
Number of pages	12
Journal	Current Genetics
Volume	46
Issue number	1
DOIs	https://doi.org/10.1007/s00294-004-0508-6
Publication status	Published - Jul 2004
Externally published	Yes

Keywords

Carotenoid
Fusarin
Fusarium
Gibberella
Opsin
Photoinduction

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title = "A gene of the opsin family in the carotenoid gene cluster of Fusarium fujikuroi",

abstract = "Opsins are membrane photoreceptors closely related to the heat-shock proteins of the HSP30 family. Their functions include light-driven ion pumping in archaea and light detection in algae and animals, using the apocarotenoid retinal as a light-absorbing prosthetic group. We describe a gene of Fusarium fujikuroi, carO, coding for a polypeptide resembling opsins and HSP30-like proteins and contiguous to the genes of the carotenoid pathway, carRA and carB. Transcription of carO is induced by light and is deregulated in carotenoid-overproducing mutants. The same regulation pattern is exhibited by carRA and carB; and common conserved DNA elements are found in the three promoters. Heat shock resulted in a modest induction of carO transcription, similar to the one exhibited by carB, confirming a common regulation. Targeted mutagenesis of carO produced no apparent phenotypic modification, including no change in the photoinduction of carotenoid biosynthesis.",

keywords = "Carotenoid, Fusarin, Fusarium, Gibberella, Opsin, Photoinduction",

author = "Prado, {Maria M.} and Alfonso Prado-Cabrero and Rafael Fern{\'a}ndez-Mart{\'i}n and Javier Avalos",

note = "Funding Information: Acknowledgements We thank M.I.G. Roncero and M.D. Garc{\'i}a-Pedrajas for information on the protoplast preparation method, E. Cerd{\'a}-Olmedo for useful discussions and C. Vallejo and L. P{\'e}rez de Camino for technical assistance. This work was supported by the European Union (project QLK1-CT-2001-00780) and the Spanish Government (INIA, project PB96-1336).",

year = "2004",

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doi = "10.1007/s00294-004-0508-6",

language = "English",

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journal = "Current Genetics",

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AU - Prado, Maria M.

AU - Prado-Cabrero, Alfonso

AU - Fernández-Martín, Rafael

AU - Avalos, Javier

N1 - Funding Information: Acknowledgements We thank M.I.G. Roncero and M.D. García-Pedrajas for information on the protoplast preparation method, E. Cerdá-Olmedo for useful discussions and C. Vallejo and L. Pérez de Camino for technical assistance. This work was supported by the European Union (project QLK1-CT-2001-00780) and the Spanish Government (INIA, project PB96-1336).

PY - 2004/7

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N2 - Opsins are membrane photoreceptors closely related to the heat-shock proteins of the HSP30 family. Their functions include light-driven ion pumping in archaea and light detection in algae and animals, using the apocarotenoid retinal as a light-absorbing prosthetic group. We describe a gene of Fusarium fujikuroi, carO, coding for a polypeptide resembling opsins and HSP30-like proteins and contiguous to the genes of the carotenoid pathway, carRA and carB. Transcription of carO is induced by light and is deregulated in carotenoid-overproducing mutants. The same regulation pattern is exhibited by carRA and carB; and common conserved DNA elements are found in the three promoters. Heat shock resulted in a modest induction of carO transcription, similar to the one exhibited by carB, confirming a common regulation. Targeted mutagenesis of carO produced no apparent phenotypic modification, including no change in the photoinduction of carotenoid biosynthesis.

AB - Opsins are membrane photoreceptors closely related to the heat-shock proteins of the HSP30 family. Their functions include light-driven ion pumping in archaea and light detection in algae and animals, using the apocarotenoid retinal as a light-absorbing prosthetic group. We describe a gene of Fusarium fujikuroi, carO, coding for a polypeptide resembling opsins and HSP30-like proteins and contiguous to the genes of the carotenoid pathway, carRA and carB. Transcription of carO is induced by light and is deregulated in carotenoid-overproducing mutants. The same regulation pattern is exhibited by carRA and carB; and common conserved DNA elements are found in the three promoters. Heat shock resulted in a modest induction of carO transcription, similar to the one exhibited by carB, confirming a common regulation. Targeted mutagenesis of carO produced no apparent phenotypic modification, including no change in the photoinduction of carotenoid biosynthesis.

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A gene of the opsin family in the carotenoid gene cluster of Fusarium fujikuroi

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Keywords

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