A gene of the opsin family in the carotenoid gene cluster of Fusarium fujikuroi

Maria M. Prado, Alfonso Prado-Cabrero, Rafael Fernández-Martín, Javier Avalos

Research output: Contribution to journalArticlepeer-review

65 Citations (Scopus)

Abstract

Opsins are membrane photoreceptors closely related to the heat-shock proteins of the HSP30 family. Their functions include light-driven ion pumping in archaea and light detection in algae and animals, using the apocarotenoid retinal as a light-absorbing prosthetic group. We describe a gene of Fusarium fujikuroi, carO, coding for a polypeptide resembling opsins and HSP30-like proteins and contiguous to the genes of the carotenoid pathway, carRA and carB. Transcription of carO is induced by light and is deregulated in carotenoid-overproducing mutants. The same regulation pattern is exhibited by carRA and carB; and common conserved DNA elements are found in the three promoters. Heat shock resulted in a modest induction of carO transcription, similar to the one exhibited by carB, confirming a common regulation. Targeted mutagenesis of carO produced no apparent phenotypic modification, including no change in the photoinduction of carotenoid biosynthesis.

Original languageEnglish
Pages (from-to)47-58
Number of pages12
JournalCurrent Genetics
Volume46
Issue number1
DOIs
Publication statusPublished - Jul 2004
Externally publishedYes

Keywords

  • Carotenoid
  • Fusarin
  • Fusarium
  • Gibberella
  • Opsin
  • Photoinduction

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