The present invention relates to an isolated nitrile-metabolising Pantoea strain deposited under NCIMB 41853 and to amide or acid-producing mutants and variants thereof. Also provided is an isolated nucleotide sequence or polypeptide obtainable from the nitrile-metabolising Pantoea strain of the invention wherein the isolated nucleotide sequence or polypeptide encodes or is a nitrilase. The strain, isolated nucleotide sequence or polypeptide of the invention may be used in a process for producing a carboxylic acid by metabolising nitriles such as 3-hydroxyglutaronitrile, 3-hydroxy-butyronitrile and 3-hydroxyphenylpropionitrile to form corresponding carboxylic acids.
|Original language||English (Ireland)|
|Publication status||Published - 07 Jul 2011|