TY - JOUR
T1 - Analysis of changes in phosphorylation of receptor tyrosine kinases
T2 - Antibody arrays
AU - Rani, Sweta
AU - O’driscoll, Lorraine
N1 - Publisher Copyright:
© Springer Science+Business Media New York 2015.
PY - 2015
Y1 - 2015
N2 - Tyrosine kinases are mainly classifi ed into two groups, as receptor tyrosine kinase (RTK) and non-receptor tyrosine kinase (NRTK). The RTK family of transmembrane ligand-binding proteins are important mediators of the signaling cascade and includes EGFR, PDGFR (platelet-derived growth factor receptors), FGFR (fi broblast growth factor receptor) and the IR (insulin receptor). RTKs comprise 59 members and their structure includes an extracellular ligand-binding domain, a transmembrane domain, and an intracellular domain possessing the tyrosine kinase activity. This chapter focuses on antibody arrays that are basically used to analyse phosphorylation and dephosphorylation of RTKs. Antibody arrays include well-characterized antibodies for profi ling, changes in RTK expression, and comparison between normal, diseased, or treated samples.
AB - Tyrosine kinases are mainly classifi ed into two groups, as receptor tyrosine kinase (RTK) and non-receptor tyrosine kinase (NRTK). The RTK family of transmembrane ligand-binding proteins are important mediators of the signaling cascade and includes EGFR, PDGFR (platelet-derived growth factor receptors), FGFR (fi broblast growth factor receptor) and the IR (insulin receptor). RTKs comprise 59 members and their structure includes an extracellular ligand-binding domain, a transmembrane domain, and an intracellular domain possessing the tyrosine kinase activity. This chapter focuses on antibody arrays that are basically used to analyse phosphorylation and dephosphorylation of RTKs. Antibody arrays include well-characterized antibodies for profi ling, changes in RTK expression, and comparison between normal, diseased, or treated samples.
KW - Cell lysis
KW - Kinase activity
KW - Phosphorylation
KW - Protein arrays
KW - Receptor tyrosine kinase
UR - http://www.scopus.com/inward/record.url?scp=84955385777&partnerID=8YFLogxK
U2 - 10.1007/978-1-4939-1789-1_2
DO - 10.1007/978-1-4939-1789-1_2
M3 - Article
C2 - 25319885
AN - SCOPUS:84955385777
VL - 1233
SP - 15
EP - 23
JO - Methods in Molecular Biology
JF - Methods in Molecular Biology
SN - 1064-3745
ER -