Analysis of changes in phosphorylation of receptor tyrosine kinases: Antibody arrays

Sweta Rani, Lorraine O’driscoll

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)

Abstract

Tyrosine kinases are mainly classifi ed into two groups, as receptor tyrosine kinase (RTK) and non-receptor tyrosine kinase (NRTK). The RTK family of transmembrane ligand-binding proteins are important mediators of the signaling cascade and includes EGFR, PDGFR (platelet-derived growth factor receptors), FGFR (fi broblast growth factor receptor) and the IR (insulin receptor). RTKs comprise 59 members and their structure includes an extracellular ligand-binding domain, a transmembrane domain, and an intracellular domain possessing the tyrosine kinase activity. This chapter focuses on antibody arrays that are basically used to analyse phosphorylation and dephosphorylation of RTKs. Antibody arrays include well-characterized antibodies for profi ling, changes in RTK expression, and comparison between normal, diseased, or treated samples.

Original languageEnglish
Pages (from-to)15-23
Number of pages9
JournalMethods in Molecular Biology
Volume1233
DOIs
Publication statusPublished - 2015

Keywords

  • Cell lysis
  • Kinase activity
  • Phosphorylation
  • Protein arrays
  • Receptor tyrosine kinase

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