Analysis of changes in phosphorylation of receptor tyrosine kinases: Antibody arrays

Sweta Rani, Lorraine O'Driscoll

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

4 Citations (Scopus)

Abstract

Tyrosine kinases are mainly classified into two groups, as receptor tyrosine kinase (RTK) and non-receptor tyrosine kinase (NRTK). The RTK family of transmembrane ligand-binding proteins are important mediators of the signaling cascade and includes EGFR, PDGFR (platelet-derived growth factor receptors), FGFR (fibroblast growth factor receptor) and the IR (insulin receptor). RTKs comprise 59 members and their structure includes an extracellular ligand-binding domain, a transmembrane domain, and an intracellular domain possessing the tyrosine kinase activity. This chapter focuses on antibody arrays that are basically used to analyse phosphorylation and dephosphorylation of RTKs. Antibody arrays include well-characterized antibodies for profiling, changes in RTK expression, and comparison between normal, diseased, or treated samples.

Original languageEnglish
Title of host publicationReceptor Tyrosine Kinases
Subtitle of host publicationMethods and Protocols
PublisherSpringer
Pages15-23
Number of pages9
ISBN (Electronic)9781493917891
ISBN (Print)9781493917884
DOIs
Publication statusPublished - 15 Oct 2014
Externally publishedYes

Keywords

  • Cell lysis
  • Kinase Activity
  • Phosphorylation
  • Protein arrays
  • Receptor tyrosine kinase

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