Assessment of lutein, zeaxanthin and meso-zeaxanthin concentrations in dietary supplements by chiral high-performance liquid chromatography

Alfonso Prado-Cabrero, Stephen Beatty, Alan Howard, Jim Stack, Philipp Bettin, John M. Nolan

Research output: Contribution to journalArticlepeer-review

15 Citations (Scopus)

Abstract

We investigated the concordance between actual and declared content of the three macular carotenoids in commercially available supplements aimed at eye health. Three batches of nine products were tested for content of lutein (L), zeaxanthin (Z) and meso-zeaxanthin (MZ) by chiral HPLC–DAD. In every product tested, actual L concentration was close to target, but Z concentration varied greatly (47–248 % of declared concentration), and the L:Z ratio within some supplements was adversely affected in consequence. In six of seven products not declaring MZ, we found this carotenoid, and four of them, using the same L source, contained a concentration of MZ that correlated positively and significantly with measured concentrations of L (r2 = 0.86; P < 0.001). More transparency is needed in terms of concordance between actual and declared concentrations of Z in commercially available formulations, and MZ should be declared in those formulations where it is present.

Original languageEnglish
Pages (from-to)599-608
Number of pages10
JournalEuropean Food Research and Technology
Volume242
Issue number4
DOIs
Publication statusPublished - 01 Apr 2016

Keywords

  • Chiral HPLC–DAD
  • Lutein
  • Macula
  • meso-zeaxanthin
  • Zeaxanthin

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