Assessment of the antioxidant and antibacterial activities of different olive processing wastewaters

Majdouline Belaqziz, Shiau Pin Tan, Abdelilah El-Abbassi, Hajar Kiai, Abdellatif Hafidi, Orla O’Donovan, Peter McLoughlin

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44 Citations (Scopus)


Olive processing wastewaters (OPW), namely olive mill wastewater (OMW) and table-olive wastewaters (TOW) were evaluated for their antibacterial activity against five Gram-positive and two Gram-negative bacteria using the standard disc diffusion and thin layer chromatography (TLC)-bioautography assays. Disc diffusion screening and bioautography of OMW were compared to the phenolic extracts of table-olive brines. Positive activity against S. aureus was demonstrated. The optimization of chromatographic separation revealed that hexane/acetone in the ratio of 4:6 was the most effective for phenolic compounds separation. A HPLC-MS analysis was performed showing that only two compounds, hydroxytyrosol and tyrosol, were the predominant phenolic compounds in all OPW. The phenolic extract of OMW generated by a semi-modern process showed the highest free radical-scavenging activity (DPPH assay) compared to the other phenolic extracts. It is apparent from the present study that OPW are a rich source of antioxidants suitable for use in food, cosmetic or pharmaceutical applications.

Original languageEnglish
Article numbere0182622
JournalPLoS ONE
Issue number9
Publication statusPublished - Sep 2017


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