Atrazine analysis using an amperometric immunosensor based on single-chain antibody fragments and regeneration-free multi-calibrant measurement

Kathleen Grennan, Gillian Strachan, Andrew J. Porter, Anthony J. Killard, Malcolm R. Smyth

Research output: Contribution to journalArticlepeer-review

93 Citations (Scopus)

Abstract

This work describes the development of an electrochemical immunosensor for the analysis of atrazine using recombinant single-chain antibody (scAb) fragments. The sensors are based on carbon paste screen-printed electrodes incorporating the conducting polymer polyaniline (PANI)/poly(vinylsulphonic acid) (PVSA), which enables direct mediatorless coupling to take place between the redox centres of antigen-labelled horseradish peroxidase (HRP) and the electrode surface. Competitive immunoassays can be performed in real-time using this separation-free system. Analytical measurements based on the pseudo-linear relationship between the slope of a real-time amperometric signal and the concentration of analyte, yield a novel immunosensor set-up capable of regenerationless amperometric analysis. Multiple, sequential measurements of standards and samples can be performed on a single scAb-modified surface in a matter of minutes. No separation of bound and unbound species was necessary prior to detection. The system is capable of measuring atrazine to a detection limit of 0.1ppb (0.1μgl-1). This system offers the potential for rapid, cost-effective immunosensing for the analysis of samples of environmental, medical and pharmaceutical significance.

Original languageEnglish
Pages (from-to)287-298
Number of pages12
JournalAnalytica Chimica Acta
Volume500
Issue number1-2
DOIs
Publication statusPublished - 19 Dec 2003
Externally publishedYes

Keywords

  • Amperometric
  • Atrazine
  • Multi-calibrant analysis
  • Scab
  • Screen-printed electrode

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