Cloning and expression of the carbon monoxide dehydrogenase genes from Pseudomonas thermocarboxydovorans strain C2

Gary W. Black, Catherine M. Lyons, Edwin Williams, John Colby, Michael Kehoe, Catherine O'Reilly

Research output: Contribution to journalArticlepeer-review

12 Citations (Scopus)

Abstract

Carbon monoxide dehydrogenase (CODH) from Pseudomonas thermocarboxydovorans strain C2 is composed of three non-identical subunits. A gene library of C2 DNA in lambda vector L47.1 was generated and screened using anti-CODH serum. Western blotting experiments revealed a protein which co-migrated with and had the same immunological reaction as the large subunit of CODH in some of the clones isolated from the library. The coding region was pinpointed to a 4 kb fragment which was subcloned into plasmid. Western blotting experiments showed that all three subunits of CODH were coded for by the subclone. However, no CODH activity was detected.

Original languageEnglish
Pages (from-to)249-254
Number of pages6
JournalFEMS Microbiology Letters
Volume70
Issue number3
DOIs
Publication statusPublished - Aug 1990
Externally publishedYes

Keywords

  • Carbon monoxide
  • Carbon monoxide dehydrogenase
  • Carboxydotrophic bacterium
  • Cloning
  • Expression
  • Immunological screening
  • Pseudomonas thermocarboxydovorans C2
  • Restriction analysis
  • Western blot analysis

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