Cloning and expression of the carbon monoxide dehydrogenase genes from Pseudomonas thermocarboxydovorans strain C2

Gary W. Black; Catherine M. Lyons; Edwin Williams; John Colby; Michael Kehoe; Catherine O'Reilly

doi:10.1016/S0378-1097(05)80003-5

Cloning and expression of the carbon monoxide dehydrogenase genes from Pseudomonas thermocarboxydovorans strain C2

Gary W. Black, Catherine M. Lyons, Edwin Williams, John Colby, Michael Kehoe, Catherine O'Reilly

Research output: Contribution to journal › Article › peer-review

12 Citations (Scopus)

Abstract

Carbon monoxide dehydrogenase (CODH) from Pseudomonas thermocarboxydovorans strain C2 is composed of three non-identical subunits. A gene library of C2 DNA in lambda vector L47.1 was generated and screened using anti-CODH serum. Western blotting experiments revealed a protein which co-migrated with and had the same immunological reaction as the large subunit of CODH in some of the clones isolated from the library. The coding region was pinpointed to a 4 kb fragment which was subcloned into plasmid. Western blotting experiments showed that all three subunits of CODH were coded for by the subclone. However, no CODH activity was detected.

Original language	English
Pages (from-to)	249-254
Number of pages	6
Journal	FEMS Microbiology Letters
Volume	70
Issue number	3
DOIs	https://doi.org/10.1016/S0378-1097(05)80003-5
Publication status	Published - Aug 1990
Externally published	Yes

Keywords

Carbon monoxide
Carbon monoxide dehydrogenase
Carboxydotrophic bacterium
Cloning
Expression
Immunological screening
Pseudomonas thermocarboxydovorans C2
Restriction analysis
Western blot analysis

Access to Document

10.1016/S0378-1097(05)80003-5

Cite this

@article{8a178123ad564f0aa040a5af779870bc,

title = "Cloning and expression of the carbon monoxide dehydrogenase genes from Pseudomonas thermocarboxydovorans strain C2",

abstract = "Carbon monoxide dehydrogenase (CODH) from Pseudomonas thermocarboxydovorans strain C2 is composed of three non-identical subunits. A gene library of C2 DNA in lambda vector L47.1 was generated and screened using anti-CODH serum. Western blotting experiments revealed a protein which co-migrated with and had the same immunological reaction as the large subunit of CODH in some of the clones isolated from the library. The coding region was pinpointed to a 4 kb fragment which was subcloned into plasmid. Western blotting experiments showed that all three subunits of CODH were coded for by the subclone. However, no CODH activity was detected.",

keywords = "Carbon monoxide, Carbon monoxide dehydrogenase, Carboxydotrophic bacterium, Cloning, Expression, Immunological screening, Pseudomonas thermocarboxydovorans C2, Restriction analysis, Western blot analysis",

author = "Black, {Gary W.} and Lyons, {Catherine M.} and Edwin Williams and John Colby and Michael Kehoe and Catherine O'Reilly",

year = "1990",

month = aug,

doi = "10.1016/S0378-1097(05)80003-5",

language = "English",

volume = "70",

pages = "249--254",

journal = "FEMS Microbiology Letters",

issn = "0378-1097",

publisher = "Oxford University Press",

number = "3",

}

TY - JOUR

T1 - Cloning and expression of the carbon monoxide dehydrogenase genes from Pseudomonas thermocarboxydovorans strain C2

AU - Black, Gary W.

AU - Lyons, Catherine M.

AU - Williams, Edwin

AU - Colby, John

AU - Kehoe, Michael

AU - O'Reilly, Catherine

PY - 1990/8

Y1 - 1990/8

N2 - Carbon monoxide dehydrogenase (CODH) from Pseudomonas thermocarboxydovorans strain C2 is composed of three non-identical subunits. A gene library of C2 DNA in lambda vector L47.1 was generated and screened using anti-CODH serum. Western blotting experiments revealed a protein which co-migrated with and had the same immunological reaction as the large subunit of CODH in some of the clones isolated from the library. The coding region was pinpointed to a 4 kb fragment which was subcloned into plasmid. Western blotting experiments showed that all three subunits of CODH were coded for by the subclone. However, no CODH activity was detected.

AB - Carbon monoxide dehydrogenase (CODH) from Pseudomonas thermocarboxydovorans strain C2 is composed of three non-identical subunits. A gene library of C2 DNA in lambda vector L47.1 was generated and screened using anti-CODH serum. Western blotting experiments revealed a protein which co-migrated with and had the same immunological reaction as the large subunit of CODH in some of the clones isolated from the library. The coding region was pinpointed to a 4 kb fragment which was subcloned into plasmid. Western blotting experiments showed that all three subunits of CODH were coded for by the subclone. However, no CODH activity was detected.

KW - Carbon monoxide

KW - Carbon monoxide dehydrogenase

KW - Carboxydotrophic bacterium

KW - Cloning

KW - Expression

KW - Immunological screening

KW - Pseudomonas thermocarboxydovorans C2

KW - Restriction analysis

KW - Western blot analysis

UR - http://www.scopus.com/inward/record.url?scp=0025345938&partnerID=8YFLogxK

U2 - 10.1016/S0378-1097(05)80003-5

DO - 10.1016/S0378-1097(05)80003-5

M3 - Article

C2 - 2227359

AN - SCOPUS:0025345938

VL - 70

SP - 249

EP - 254

JO - FEMS Microbiology Letters

JF - FEMS Microbiology Letters

SN - 0378-1097

IS - 3

ER -

Cloning and expression of the carbon monoxide dehydrogenase genes from Pseudomonas thermocarboxydovorans strain C2

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this