Abstract
Carbon monoxide dehydrogenase (CODH) from Pseudomonas thermocarboxydovorans strain C2 is composed of three non-identical subunits. A gene library of C2 DNA in lambda vector L47.1 was generated and screened using anti-CODH serum. Western blotting experiments revealed a protein which co-migrated with and had the same immunological reaction as the large subunit of CODH in some of the clones isolated from the library. The coding region was pinpointed to a 4 kb fragment which was subcloned into plasmid. Western blotting experiments showed that all three subunits of CODH were coded for by the subclone. However, no CODH activity was detected.
Original language | English |
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Pages (from-to) | 249-254 |
Number of pages | 6 |
Journal | FEMS Microbiology Letters |
Volume | 70 |
Issue number | 3 |
DOIs | |
Publication status | Published - Aug 1990 |
Externally published | Yes |
Keywords
- Carbon monoxide
- Carbon monoxide dehydrogenase
- Carboxydotrophic bacterium
- Cloning
- Expression
- Immunological screening
- Pseudomonas thermocarboxydovorans C2
- Restriction analysis
- Western blot analysis