Development of a probiotic Cheddar cheese containing human-derived Lactobacillus paracasei strains

G. Gardiner, R. P. Ross, J. K. Collins, G. Fitzgerald, C. Stanton

Research output: Contribution to journalArticlepeer-review

224 Citations (Scopus)


Cheddar cheese was manufactured with either Lactobacillus salivarius NFBC 310, NFBC 321, or NFBC 348 or L. paracasei NFBC 338 or NFBC 364 as the dairy starter adjunct. These five strains had previously been isolated from the human small intestine and have been characterized extensively with respect to their probiotic potential. Enumeration of these strains in mature Cheddar cheese, however, was complicated by the presence of high numbers (> 107 CFU/g of cheese) of nonstarter lactic acid bacteria, principally composed of lactobacilli which proliferate as the cheese ripens. Attempts to differentiate the adjunct lactobacilli from the nonstarter lactobacilli based on bile tolerance and growth temperature were unsuccessful. In contrast, the randomly amplified polymorphic DNA method allowed the generation of discrete DNA fingerprints for each strain which were clearly distinguishable from those generated from the natural flora of the cheeses. Using this approach, it was found that both L. paracasei strains grew and sustained high viability in cheese during ripening, while each of the L. salivarius species declined over the ripening period. These data demonstrate that Cheddar cheese can be an effective vehicle for delivery of some probiotic organisms to the consumer.

Original languageEnglish
Pages (from-to)2192-2199
Number of pages8
JournalApplied and Environmental Microbiology
Issue number6
Publication statusPublished - Jun 1998
Externally publishedYes


Dive into the research topics of 'Development of a probiotic Cheddar cheese containing human-derived Lactobacillus paracasei strains'. Together they form a unique fingerprint.

Cite this