Echinenone vibrational properties: From solvents to the orange carotenoid protein

Elizabeth Kish, Maria Manuela Mendes Pinto, Diana Kirilovsky, Riccardo Spezia, Bruno Robert

Research output: Contribution to journalArticlepeer-review

34 Citations (Scopus)

Abstract

Abstract Orange carotenoid protein (OCP) is a cyanobacterial photoactive protein which binds echinenone as a chromophore; it is involved in photoprotection of these photosynthetic organisms against intense illumination. In its resting state, OCP appears orange (OCPo), and turns into a red form (OCPr) when exposed to blue-green light. Here we have combined resonance Raman spectroscopy and molecular modeling to investigate the mechanisms underlying the electronic absorption properties of the different forms of OCP. Our results show that there are at least two carotenoid configurations in the OCPo, suggesting that it is quite flexible, and that the OCPo to OCPr transition must involve an increase of the apparent conjugation length of the bound echinenone. Resonance Raman indicates that this chromophore must be in an all-trans configuration in OCPo. Density functional theory (DFT) calculations, in agreement with the Raman spectra of both OCP forms, show that the OCPo to OCPr transition must involve either an echinenone s-cis to s-trans isomerization which would affect the position of its conjugated end-chain rings, or a bending of the echinenone rings which would bring them from out of the plane of the CC conjugated plane in the OCPo form into the CC plane in the OCPr form.

Original languageEnglish
Article number47468
Pages (from-to)1044-1054
Number of pages11
JournalBBA - Bioenergetics
Volume1847
Issue number10
DOIs
Publication statusPublished - 25 Jun 2015
Externally publishedYes

Keywords

  • Biophysics
  • Carotenoid
  • Density functional theory
  • Orange carotenoid protein (OCP)
  • Photoprotection
  • Raman spectroscopy

Fingerprint

Dive into the research topics of 'Echinenone vibrational properties: From solvents to the orange carotenoid protein'. Together they form a unique fingerprint.

Cite this