The aim of this interdisciplinary study was to investigate the effects of a range of static magnetic fields (up to 5 T) on intracellular signalling cascades in primary rat cultured cortical neurons. Magnetic field exposures were conducted using a superconducting magnet with primary rat cultured cortical neurons maintained at 37°C. A series of test were carried out to investigate the effect of magnetic field strength on the activation of the proteins kinases, extracellular-signal regulated kinase (ERK) and c-Jun N terminal kinase (JNK). Cells were exposed to the magnetic fields for one hour, fixed in 4% paraformaldehyde and examined for ERK and JNK activation using antibodies which recognise the phosphorylated (i.e., active) form of ERK or JNK. This immunocytochemical data demonstrate a differential activation of ERK and JNK when cells are exposed to increasing magnetic field. Thus, ERK activation was increased when cells were exposed to 0.75 T whereas JNK activation was increased at a higher magnetic field strength of 5 T. Since ERK and JNK have been implicated in cellular differentiation and stress response, respectively, the results presented here suggest that static magnetic field strength may influence those cellular parameters.
|Number of pages||3|
|Publication status||Published - 2005|
|Event||1st International Meeting on Applied Physics, APHYS-2003 - Badajoz, Spain|
Duration: 13 Oct 2003 → 18 Oct 2003