Effects of static magnetic fields on primary cortical neurons

A. Prina Mello, E. Farrell, P. J. Prendergast, V. Campbell, J. M.D. Coey

Research output: Contribution to journalConference articlepeer-review

5 Citations (Scopus)

Abstract

The aim of this interdisciplinary study was to investigate the effects of a range of static magnetic fields (up to 5 T) on intracellular signalling cascades in primary rat cultured cortical neurons. Magnetic field exposures were conducted using a superconducting magnet with primary rat cultured cortical neurons maintained at 37°C. A series of test were carried out to investigate the effect of magnetic field strength on the activation of the proteins kinases, extracellular-signal regulated kinase (ERK) and c-Jun N terminal kinase (JNK). Cells were exposed to the magnetic fields for one hour, fixed in 4% paraformaldehyde and examined for ERK and JNK activation using antibodies which recognise the phosphorylated (i.e., active) form of ERK or JNK. This immunocytochemical data demonstrate a differential activation of ERK and JNK when cells are exposed to increasing magnetic field. Thus, ERK activation was increased when cells were exposed to 0.75 T whereas JNK activation was increased at a higher magnetic field strength of 5 T. Since ERK and JNK have been implicated in cellular differentiation and stress response, respectively, the results presented here suggest that static magnetic field strength may influence those cellular parameters.

Original languageEnglish
Pages (from-to)205-207
Number of pages3
JournalPhysica Scripta
VolumeT118
DOIs
Publication statusPublished - 2005
Externally publishedYes
Event1st International Meeting on Applied Physics, APHYS-2003 - Badajoz, Spain
Duration: 13 Oct 200318 Oct 2003

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