Efficient species identification of pine marten (Martes martes) and red fox (Vulpes vulpes) scats using a 5′ nuclease real-time PCR assay

Catherine O'Reilly, Mark Statham, Jacinta Mullins, Peter D. Turner, Declan O'Mahony

Research output: Contribution to journalArticlepeer-review

30 Citations (Scopus)

Abstract

Monitoring wildlife species by DNA identification of samples collected non-invasively is an important tool in conservation management. DNA identification of species from faecal (scat) samples is problematic due to the small quantities and poor quality of the DNA isolated from such samples. This study demonstrates the use of real-time PCR technology in the identification of red fox (Vulpes vulpes) and pine marten (Martes martes). It is shown that real-time PCR can be used to identify fox and pine marten by either melting curve analysis (Tm determination) with SYBR Green 1 detection or by the use of species specific fluorogenic probes. The technique is shown to work efficiently with scat DNA.

Original languageEnglish
Pages (from-to)735-738
Number of pages4
JournalConservation Genetics
Volume9
Issue number3
DOIs
Publication statusPublished - Jun 2008

Keywords

  • Fox
  • Pine marten
  • Real-time PCR
  • Scat
  • Species identification
  • TaqMan probes

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