Evaluation of Some Carotenoids in Grapes by Reversed]- and Normal-Phase Liquid Chromatography: A Qualitative Analysis

Maria Manuela Mendes-Pinto, A. C.Silva Ferreria, M. Beatriz P.P. Oliveira, Paula Guedes De Pinho

Research output: Contribution to journalArticlepeer-review

40 Citations (Scopus)

Abstract

Carotenoids in grapes of three Port winemaking cultivars were investigated. Extracts were obtained with n-hexane/diethyl ether mixtures (0/100; 20/80; 50/50; 100/0) and analyzed by normal and reversed phase HPLC-DAD. Selection and identification of peaks were based on spectroscopic characteristics - λmax, (%III/II) and k′ values, leading to 28 probable carotenoids. Using pure standards, it was possible to identify seven compounds previously described (neochrome, neoxanthin, violaxanthin, flavoxanthin, zeaxanthin, lutein, and β-carotene), one more type of neochrome reported here, for the first time, and in addition, two geometrical isomers of lutein and β-carotene were tentatively described. The remaining 17 need to be further identified. High polarity solvent mixtures lead to qualitatively richer chromatograms. Reversed-phase separations allowed the detection of flavoxanthin and the possible geometrical isomer(s) of β-carotene. Under normal phase, zeaxanthin was detected, and neochromes were better separated from neoxanthin. Extraction with 50/50 n-hexane/diethyl ether mixtures and reversed-phase conditions was the best combination for analysis of the carotenoids, known as precursors of compounds with high aroma impact in wines.

Original languageEnglish
Pages (from-to)3182-3188
Number of pages7
JournalJournal of Agricultural and Food Chemistry
Volume52
Issue number10
DOIs
Publication statusPublished - 19 May 2004
Externally publishedYes

Keywords

  • Caratenoid identification
  • Wine grapes

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