Excited state complex formation between 3-aminophthalhydrazide and DNA: A fluorescence quenching reaction

G. Will, E. Kudryashov, E. Duggan, D. Fitzmaurice, V. Buckin, E. Waghorne, S. Mukherjee

Research output: Contribution to journalArticlepeer-review

13 Citations (Scopus)

Abstract

Fluorescence quenching reaction of 3-aminophthalhyrdazide (luminol) due to the interaction with short DNA fragments has been studied employing steady-state and time correlated single photon counting techniques. The interaction of luminol with DNA in the excited state has also been analyzed using Stern-Volmer (S-V) mechanism. A very weak ground state interaction is proposed to explain the higher values of the quenching rate constant and is consistent with positive curvatures in the S-V plots. A plausible explanation of the quenching mechanism has been discussed on the basis of hydrogen bonding interaction between luminol and DNA fragment. It is proposed that the phosphate backbone present in DNA is responsible for the interaction with luminol.

Original languageEnglish
Pages (from-to)2711-2717
Number of pages7
JournalSpectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy
Volume55
Issue number13
DOIs
Publication statusPublished - Nov 1999
Externally publishedYes

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