Fluorescence quenching reaction of 3-aminophthalhyrdazide (luminol) due to the interaction with short DNA fragments has been studied employing steady-state and time correlated single photon counting techniques. The interaction of luminol with DNA in the excited state has also been analyzed using Stern-Volmer (S-V) mechanism. A very weak ground state interaction is proposed to explain the higher values of the quenching rate constant and is consistent with positive curvatures in the S-V plots. A plausible explanation of the quenching mechanism has been discussed on the basis of hydrogen bonding interaction between luminol and DNA fragment. It is proposed that the phosphate backbone present in DNA is responsible for the interaction with luminol.
|Number of pages||7|
|Journal||Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy|
|Publication status||Published - Nov 1999|