Fast separation of UV absorbing anions using ion-interaction chromatography

Damian Connolly, Brett Paull

Research output: Contribution to journalArticlepeer-review

30 Citations (Scopus)

Abstract

Ion-interaction chromatography on a short (30×4.6 mm) 3 μm ODS column has been investigated with the aim of developing fast chromatographic separations of selected inorganic anions. Tetrabutylammonium chloride (TBA-Cl) was used as the ion-interaction reagent in mobile phases that also contained up to 20% methanol. Separations of simple test mixtures of up to eight UV absorbing anions illustrated how excellent efficiencies (>50 000 plates/m) could be obtained under optimized conditions. The use of an optimised mobile phase containing 20 mM TBA-Cl and 20% methanol resulted in the baseline separation of five important anions (iodate, bromate, nitrite, bromide and nitrate) in a separation window of just 28 s, with a shortest total analysis time of 50 s. The method was briefly applied to the rapid analysis of nitrite and nitrate in both a drinking water and a river water sample with a view to future on-line monitoring.

Original languageEnglish
Pages (from-to)353-359
Number of pages7
JournalJournal of Chromatography A
Volume917
Issue number1-2
DOIs
Publication statusPublished - 11 May 2001
Externally publishedYes

Keywords

  • Fast ion-interaction chromatography
  • Inorganic anions

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