High-performance separation of small inorganic anions on a methacrylate-based polymer monolith grafted with [2(methacryloyloxy)ethyl] trimethylammonium chloride

Damian Connolly, Brett Paull

Research output: Contribution to journalArticlepeer-review

23 Citations (Scopus)

Abstract

A glycidyl methacrylate-co-ethylene dimethacrylate monolith in capillary format (100 μm/id) has been grafted with chains of poly([2(methacryloyloxy) ethyl] trimethylammonium chloride (poly-META) and applied to the ion-chromatographic separation of selected inorganic anions. Grafting chains of META onto the generic monolithic scaffold resulted in a monolith with ,electrolyte responsive flow permeability', which manifested as increased permeability in the presence of electrolyte solutions. Using an eluent of 2 mM sodium benzoate and on-column contactless conductivity detection, a test mixture of six common anions was isocratically separated and detected within 12 min, with the first four anions baseline resolved within a retention time window of 3.2 min. Retention time precision was ≤1.2% for all anions tested. Separation efficiencies of 15000 N/m were achieved for fluoride at 1 μL/min, with column efficiencies up to 29500 N/m obtained at a lower flow rate of 100 nL/min. Furthermore, repeatability of the column modification procedure using photografting methods was acceptable, with retention times between replicate columns matching within 9%.

Original languageEnglish
Pages (from-to)2653-2658
Number of pages6
JournalJournal of Separation Science
Volume32
Issue number15-16
DOIs
Publication statusPublished - 2009
Externally publishedYes

Keywords

  • Anions
  • Capillary
  • Ion chromatography
  • Monolith
  • Photografting

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