IL-17 and IL-22 elicited by a DNA vaccine encoding ROP13 associated with protection against Toxoplasma gondii in BALB/c mice

Paria Alizadeh, Ehsan Ahmadpour, Ahmad Daryani, Tohid Kazemi, Adel Spotin, Mahmoud Mahami-Oskouei, Robin J. Flynn, Yaghob Azadi, Saba Rajabi, Siamak Sandoghchian

Research output: Contribution to journalArticlepeer-review

8 Citations (Scopus)

Abstract

Toxoplasma gondii, an intracellular parasitic protozoan, is capable of infecting man and all warm-blooded animals. Cell-mediated immunity is vital in mounting protective responses against T. gondii infection. Recent studies have shown that T-helper (Th) 17 responses may play a key role in parasite control. In this current study, we constructed a DNA vaccine encoding T. gondii ROP13 in a pcDNA vector. Groups of BALB/c mice were immunized intramuscularly with pcROP13 or controls and challenged with the RH strain of T. gondii. The results showed that immunization with pcROP13 could elicit an antibody response against T. gondii. The expression of the canonical Th17 cytokines, interleukin (IL)-17 and IL-22, were significantly increased after immunization with pcROP13 compared with control groups (p < 0.05). Furthermore, vaccination resulted in a significant decrease in parasite load (p < 0.05). The induction of Th17 related cytokines, using a ROP13 DNA vaccine, against T. gondii should be considered as a potential vaccine approach for the control of toxoplasmosis.

Original languageEnglish
Pages (from-to)10782-10788
Number of pages7
JournalJournal of Cellular Physiology
Volume234
Issue number7
DOIs
Publication statusPublished - Jul 2019
Externally publishedYes

Keywords

  • DNA vaccine
  • gene expression
  • IL-22
  • interleukin (IL)-17
  • ROP13
  • T-helper (Th) 17
  • Toxoplasma gondii

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