MAGE-D4B is a novel marker of poor prognosis and potential therapeutic target involved in breast cancer tumorigenesis

Serena Germano, Susan Kennedy, Sweta Rani, Grainne Gleeson, Martin Clynes, Padraig Doolan, Susan McDonnell, Linda Hughes, John Crown, Lorraine O'Driscoll

Research output: Contribution to journalArticlepeer-review

27 Citations (Scopus)

Abstract

Melanoma-associated antigen (MAGE) family members are generally described as tumor-specific antigens. An association between MAGE-D4B and breast cancer has yet to be reported and the functional role of the encoded protein has never been established. We performed microarray analysis of 104 invasive breast tumors and matched non-cancerous breast biopsies. qPCR was used for validation in an independent biobank. To investigate the biological relevance of MAGE-D4B in breast tumorigenesis, its phenotypic effects were assessed in vitro. Overall, MAGE-D4B was detected in 43% of tumors while undetected in normal breast tissue. MAGE-D4B was found to correlate with tumor progression and to be an independent prognostic marker for poor outcome in term of relapse-free and overall survival, with potential predictive relevance in relation to response to chemotherapy. RNA interference-mediated knockdown of MAGE-D4B significantly hampered the invasive properties of Hs578T cells by affecting anchorage-independent growth, adhesion, migration and invasion affecting anchorage-independent growth, adhesion, migration and invasion and by modulating expression of invasion-suppressor gene E-cadherin.

Original languageEnglish
Pages (from-to)1991-2002
Number of pages12
JournalInternational Journal of Cancer
Volume130
Issue number9
DOIs
Publication statusPublished - 01 May 2012
Externally publishedYes

Keywords

  • biomarker
  • breast cancer
  • MAGE-D4B
  • therapeutic target
  • tumorigenesis

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