TY - JOUR
T1 - Methotrexate-loaded biodegradable nanoparticles
T2 - Preparation, characterization and evaluation of its cytotoxic potential against U-343 MGa human neuronal glioblastoma cells
AU - Musmade, Kranti P.
AU - Deshpande, Praful B.
AU - Musmade, Prashant B.
AU - Maliyakkal, M. Naseer
AU - Kumar, A. Ranjith
AU - Reddy, M. Sreenivasa
AU - Udupa, N.
N1 - Funding Information:
The authors would like to acknowledge research grant from the Department of Biotechnology, Ministry of Science and Technology, Government of India (Project no. BT/PR10830/NNT/28/114/2008), for providing financial support.
PY - 2014/6
Y1 - 2014/6
N2 - Nanoparticles represent one of the attractive alternatives in the effective treatment of cancer chemotherapy. In the present work, formulation and development of a novel methotrexate (MTX)-loaded biodegradable nanoparticles using poly(D,L-lactide-co-glycolide) (PLGA) was carried out. The prepared nanoparticles were evaluated for physicochemical properties such as particle size, zeta potential, release studies, etc and also evaluated for its in vitro cytotoxic potential against U-343 MGa human neuronal glioblastoma cells. Particle size of optimized formulation was < 200 nm. There was a considerable decrease in cell viability and enhancement in cytotoxic activity of MTX-loaded nanoparticles compared to MTX alone when tested against U-343 MGa human neuronal glioblastoma cells.
AB - Nanoparticles represent one of the attractive alternatives in the effective treatment of cancer chemotherapy. In the present work, formulation and development of a novel methotrexate (MTX)-loaded biodegradable nanoparticles using poly(D,L-lactide-co-glycolide) (PLGA) was carried out. The prepared nanoparticles were evaluated for physicochemical properties such as particle size, zeta potential, release studies, etc and also evaluated for its in vitro cytotoxic potential against U-343 MGa human neuronal glioblastoma cells. Particle size of optimized formulation was < 200 nm. There was a considerable decrease in cell viability and enhancement in cytotoxic activity of MTX-loaded nanoparticles compared to MTX alone when tested against U-343 MGa human neuronal glioblastoma cells.
KW - Cytotoxic activity
KW - Methotrexate
KW - Nanoparticles
KW - PLGA 50:50
UR - http://www.scopus.com/inward/record.url?scp=84903286868&partnerID=8YFLogxK
U2 - 10.1007/s12034-014-0030-5
DO - 10.1007/s12034-014-0030-5
M3 - Article
AN - SCOPUS:84903286868
VL - 37
SP - 945
EP - 951
JO - Bulletin of Materials Science
JF - Bulletin of Materials Science
SN - 0250-4707
IS - 4
ER -
