Micro-bore titanium housed polymer monoliths for reversed-phase liquid chromatography of small molecules

Ekaterina P. Nesterenko, Pavel N. Nesterenko, Damian Connolly, Flavie Lacroix, Brett Paull

Research output: Contribution to journalArticlepeer-review

28 Citations (Scopus)

Abstract

A new method for the fixation of polymethacrylate monoliths within titanium tubing of up to 0.8 mm I.D. for use as a chromatographic column under elevated temperatures and pressures is described. The preparation of butyl methacrylate-ethylene dimethacrylate-based monolithic stationary phases with desired porous structures was achieved within titanium tubing with pre-oxidised internal walls. The oxidised titanium surface was subsequently silanised with 3-trimethoxysilylpropyl methacrylate resulting in tight bonding of butyl methacrylate porous monolith to the internal walls, providing stationary phase stability at column temperatures up to 110 °C and at operating column pressure drops of >28 MPa. The titanium housed monoliths exhibited a uniform and dense porous structure, which provided peak efficiencies of up to 59,000 theoretical plates per meter when evaluated for the separation of small molecules in reversed-phase mode, under optimal conditions (achieved at 15 μL/min and temperature of 110 °C for naphthalene with a retention factor, k = 0.58). The developed column was applied to the reversed-phase isocratic separation of a text mixture of pesticides.

Original languageEnglish
Pages (from-to)2138-2146
Number of pages9
JournalJournal of Chromatography A
Volume1217
Issue number14
DOIs
Publication statusPublished - 02 Apr 2010
Externally publishedYes

Keywords

  • High pressure reversed-phase HPLC
  • Polymer monolith
  • Titanium column

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