MicroRNA profiling of exosomes isolated from biofluids and conditioned media

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

12 Citations (Scopus)

Abstract

Exosomes are membrane-bound 50-100 nm vesicles released from many cell types including normal and tumorous tissues. Exosomes transport mainly miRNAs, mRNAs, enzymes, cytokines, etc. from the cells of origin to the neighbor cells mediating the communication between them. The content of exosomes can be explored using RNA profiling after their isolation from medium conditioned by cultured cells or from other biofluids. This chapter includes detailed discussion on isolation, characterization, and miRNA profiling of exosomes. First, exosomes are isolated by filtration and ultracentrifugation, and then characterized using immunoblotting and transmission electron microscope. Finally, we used low density arrays to profile exosomal miRNA.

Original languageEnglish
Title of host publicationRNA Mapping
Subtitle of host publicationMethods and Protocols
PublisherHumana Press Inc.
Pages131-144
Number of pages14
ISBN (Print)9781493910618
DOIs
Publication statusPublished - 2014
Externally publishedYes

Publication series

NameMethods in Molecular Biology
Volume1182
ISSN (Print)1064-3745

Keywords

  • Biofluids
  • Cell line
  • Conditioned medium
  • Exosomes
  • Extracellular
  • miRNA

Fingerprint

Dive into the research topics of 'MicroRNA profiling of exosomes isolated from biofluids and conditioned media'. Together they form a unique fingerprint.

Cite this