Non-invasive genetic identification of small mammal species using real-time polymerase chain reaction

S. Moran, P. D. Turner, C. O'Reilly

Research output: Contribution to journalArticlepeer-review

16 Citations (Scopus)

Abstract

DNA identification of non-invasive samples is a potentially useful tool for monitoring small mammal species. Here we describe a novel method for identifying five small mammal species: wood mouse, bank vole, common shrew, pygmy shrew and water shrew. Species-specific real-time polymerase chain reaction primers were designed to amplify fragments of the mitochondrial cytochrome b gene from hair and scat samples. We also amplified nuclear DNA from scats, demonstrating their potential as a source of DNA for population genetic studies.

Original languageEnglish
Pages (from-to)1267-1269
Number of pages3
JournalMolecular Ecology Resources
Volume8
Issue number6
DOIs
Publication statusPublished - Nov 2008

Keywords

  • Cytochrome b
  • Non-invasive genetic sampling
  • Real-time PCR
  • Small mammals
  • Species identification

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