Polystyrene-co-divinylbenzene polyHIPE monoliths in 1.0 mm column formats for liquid chromatography

Sidratul Choudhury; Laurence Fitzhenry; Blánaid White; Damian Connolly

doi:10.3390/ma9030212

Polystyrene-co-divinylbenzene polyHIPE monoliths in 1.0 mm column formats for liquid chromatography

Sidratul Choudhury, Laurence Fitzhenry, Blánaid White, Damian Connolly

Department of Science

Research output: Contribution to journal › Article › peer-review

10 Citations (Scopus)

Abstract

The reversed phase liquid chromatographic (RP-HPLC) separation of small molecules using a polystyrene-co-divinylbenzene (PS-co-DVB) polyHIPE stationary phases housed within 1.0 mm i.d. silcosteel columns is presented within this study. A 90% PS-co-DVB polyHIPE was covalently attached to the walls of the column housing by prior wall modification with 3-(trimethoxysilyl) propyl methacrylate and could withstand operating backpressures in excess of 200 bar at a flow rate of 1.2 mL/min. Permeability studies revealed that the monolith swelled slightly in 100% acetonitrile relative to 100% water but could nevertheless be used to separate five alkylbenzenes using a flow rate of 40 μL/min (linear velocity: 0.57 mm/s). Remarkable column-to-column reproducibility is shown with retention factor variation between 2.6% and 6.1% for two separately prepared columns.

Original language	English
Article number	212
Journal	Materials
Volume	9
Issue number	3
DOIs	https://doi.org/10.3390/ma9030212
Publication status	Published - 18 Mar 2016

Keywords

Isocratic separation
Microbore
PolyHIPE
Polystyrene
Reversed phase LC
Silcosteel

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10.3390/ma9030212

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title = "Polystyrene-co-divinylbenzene polyHIPE monoliths in 1.0 mm column formats for liquid chromatography",

abstract = "The reversed phase liquid chromatographic (RP-HPLC) separation of small molecules using a polystyrene-co-divinylbenzene (PS-co-DVB) polyHIPE stationary phases housed within 1.0 mm i.d. silcosteel columns is presented within this study. A 90% PS-co-DVB polyHIPE was covalently attached to the walls of the column housing by prior wall modification with 3-(trimethoxysilyl) propyl methacrylate and could withstand operating backpressures in excess of 200 bar at a flow rate of 1.2 mL/min. Permeability studies revealed that the monolith swelled slightly in 100% acetonitrile relative to 100% water but could nevertheless be used to separate five alkylbenzenes using a flow rate of 40 μL/min (linear velocity: 0.57 mm/s). Remarkable column-to-column reproducibility is shown with retention factor variation between 2.6% and 6.1% for two separately prepared columns.",

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author = "Sidratul Choudhury and Laurence Fitzhenry and Bl{\'a}naid White and Damian Connolly",

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doi = "10.3390/ma9030212",

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AU - Choudhury, Sidratul

AU - Fitzhenry, Laurence

AU - White, Blánaid

AU - Connolly, Damian

PY - 2016/3/18

Y1 - 2016/3/18

N2 - The reversed phase liquid chromatographic (RP-HPLC) separation of small molecules using a polystyrene-co-divinylbenzene (PS-co-DVB) polyHIPE stationary phases housed within 1.0 mm i.d. silcosteel columns is presented within this study. A 90% PS-co-DVB polyHIPE was covalently attached to the walls of the column housing by prior wall modification with 3-(trimethoxysilyl) propyl methacrylate and could withstand operating backpressures in excess of 200 bar at a flow rate of 1.2 mL/min. Permeability studies revealed that the monolith swelled slightly in 100% acetonitrile relative to 100% water but could nevertheless be used to separate five alkylbenzenes using a flow rate of 40 μL/min (linear velocity: 0.57 mm/s). Remarkable column-to-column reproducibility is shown with retention factor variation between 2.6% and 6.1% for two separately prepared columns.

AB - The reversed phase liquid chromatographic (RP-HPLC) separation of small molecules using a polystyrene-co-divinylbenzene (PS-co-DVB) polyHIPE stationary phases housed within 1.0 mm i.d. silcosteel columns is presented within this study. A 90% PS-co-DVB polyHIPE was covalently attached to the walls of the column housing by prior wall modification with 3-(trimethoxysilyl) propyl methacrylate and could withstand operating backpressures in excess of 200 bar at a flow rate of 1.2 mL/min. Permeability studies revealed that the monolith swelled slightly in 100% acetonitrile relative to 100% water but could nevertheless be used to separate five alkylbenzenes using a flow rate of 40 μL/min (linear velocity: 0.57 mm/s). Remarkable column-to-column reproducibility is shown with retention factor variation between 2.6% and 6.1% for two separately prepared columns.

KW - Isocratic separation

KW - Microbore

KW - PolyHIPE

KW - Polystyrene

KW - Reversed phase LC

KW - Silcosteel

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U2 - 10.3390/ma9030212

DO - 10.3390/ma9030212

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JF - Materials

SN - 1996-1944

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M1 - 212

ER -

Polystyrene-co-divinylbenzene polyHIPE monoliths in 1.0 mm column formats for liquid chromatography

Abstract

Keywords

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