Polystyrene-co-divinylbenzene polyHIPE monoliths in 1.0 mm column formats for liquid chromatography

Sidratul Choudhury, Laurence Fitzhenry, Blánaid White, Damian Connolly

Research output: Contribution to journalArticlepeer-review

10 Citations (Scopus)

Abstract

The reversed phase liquid chromatographic (RP-HPLC) separation of small molecules using a polystyrene-co-divinylbenzene (PS-co-DVB) polyHIPE stationary phases housed within 1.0 mm i.d. silcosteel columns is presented within this study. A 90% PS-co-DVB polyHIPE was covalently attached to the walls of the column housing by prior wall modification with 3-(trimethoxysilyl) propyl methacrylate and could withstand operating backpressures in excess of 200 bar at a flow rate of 1.2 mL/min. Permeability studies revealed that the monolith swelled slightly in 100% acetonitrile relative to 100% water but could nevertheless be used to separate five alkylbenzenes using a flow rate of 40 μL/min (linear velocity: 0.57 mm/s). Remarkable column-to-column reproducibility is shown with retention factor variation between 2.6% and 6.1% for two separately prepared columns.

Original languageEnglish
Article number212
JournalMaterials
Volume9
Issue number3
DOIs
Publication statusPublished - 18 Mar 2016

Keywords

  • Isocratic separation
  • Microbore
  • PolyHIPE
  • Polystyrene
  • Reversed phase LC
  • Silcosteel

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