Separation of selected transition metals by capillary chelation ion chromatography using acetyl-iminodiacetic acid modified capillary polymer monoliths

Áine Moyna, Damian Connolly, Ekaterina Nesterenko, Pavel N. Nesterenko, Brett Paull

Research output: Contribution to journalArticlepeer-review

14 Citations (Scopus)

Abstract

Capillary housed laurylmethacrylate-co-ethylene dimethacrylate (LMA-co-EDMA) polymer monoliths were fabricated, functionalised with varying amounts of vinyl azlactone, followed by immobilisation of iminodiacetic acid (IDA), forming a range of acetyl-iminodiacetic acid (AIDA) functionalised monoliths, applied to the chelation ion chromatographic separation of selected transition and heavy metals. A number of monoliths of varying length and ligand density were prepared, resulting in increased cation retention and chromatographic resolution on those displaying the highest capacity. Ligand density and related column capacity were confirmed visually using scanning capacitively coupled contactless conductivity detection (sC4D) techniques. Column temperature studies to determine retention mechanism and the effect of temperature on the retention of Mn(II), Cd(II) and Cu(II) was investigated, showing an increase in retention with increased temperature for Cd(II) and Cu(II), whilst a decrease in retention was obtained for Mn(II). Isocratic capillary chelation ion chromatographic separations of Mn(II), Cd(II) and Cu(II) were obtained, with dual peak detection demonstrated using combined on-column C4D detection and UV-Visible detection following the post-capillary column reaction of the eluted metals with 4-(2-pyridylazo) resorcinol (PAR).

Original languageEnglish
Pages (from-to)155-163
Number of pages9
JournalJournal of Chromatography A
Volume1249
DOIs
Publication statusPublished - 03 Aug 2012
Externally publishedYes

Keywords

  • Acetyl-iminodiacetic acid
  • Chelation ion chromatography
  • Polymer monoliths
  • Transition metal ions

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