Serotonin levels in aqueous humor of patients with primary open-angle glaucoma

Vicente Zanon-Moreno, P. Melo, M. M. Mendes-Pinto, C. J. Alves, J. J. Garcia-Medina, I. Vinuesa-Silva, M. A. Moreno-Nadal, M. D. Pinazo-Duran

Research output: Contribution to journalArticlepeer-review

6 Citations (Scopus)


Purpose: Glaucoma is an optic neuropathy characterized by a high intraocular pressure (IOP), alterations in optic nerve head, and loss of visual field that could lead to bilateral blindness. Serotonin (5-HT) is a biogenic monoamine that is synthesized from hydroxylation of tryptophan and acts by three ways, dissemination, metabolism, and reuptake in synaptic cleft through specific systems of the membrane. The purpose of this study is to evaluate the 5-HT and 5-HIAA (5-hydroxiindolacetic acid) levels in the aqueous humor of patients with primary open-angle glaucoma (POAG). Methods: We performed a case-control study, and the patients recruited were classified into two groups, 1) 30 patients with POAG (GG) and 2) 30 patients with cataracts (CG), who acted as the controls. Aqueous humor samples of each patient were obtained by paracentesis at the beginning of the surgical procedures. 5-HT and 5-HIAA levels were determined by high performance liquid chromatography (HPLC) with electrochemical detection. Results: There were no statistical differences between age (71.3±7.2 years in GG, 73.5±9.0 years in CG; p=0.2581) or gender (sex ratio 0.765 in GG and 0.667 in CG). 5-HT levels were lower in GG, but this difference was not significant (p=0.820). We observed a statistically significant higher level of 5-HIAA in GG (p=0.001). The 5-HT turnover (5-HIAA/5-HT) were higher in GG than in CG (p<0.05), but the difference was not significant (P=0.598). Conclusions: The level of 5-HT was lower in GG patients, and the level of 5-HIAA was higher in GG patients than in CG patients.

Original languageEnglish
Pages (from-to)2143-2147
Number of pages5
JournalMolecular Vision
Publication statusPublished - 28 Nov 2008
Externally publishedYes


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