Stepped gradients on polymeric monolithic columns by photoinitiated grafting

Sinéad Currivan, Damian Connolly, Brett Paull

Research output: Contribution to journalArticlepeer-review

9 Citations (Scopus)


The surface of polymethacrylate monoliths was functionalized by a post-polymerization modification, by means of a novel photo-initiated graft procedure where the charged monomer, sulfopropyl methacrylate, was controllably grafted stepwise, i.e. with incremental graft energies. The grafting approach was optimized using scanning capacitively coupled contactless conductivity detection. The effect of the localized ion exchange capacity and resultant gradient stationary phase upon ion-exchange chromatographic retention, selectivity, and performance was investigated, and compared to a homogeneously grafted (isotropic) column. The gradient column provided reduced peak widths at half height for both cationic analytes, with a reduction of 34 and 33%, respectively, when compared to the isotropic column.

Original languageEnglish
Pages (from-to)3795-3802
Number of pages8
JournalJournal of Separation Science
Issue number21
Publication statusPublished - Nov 2015


  • Capacitively coupled contactless conductivity detection
  • Cation exchange
  • Gradient
  • Grafting
  • Polymer monoliths


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