The Influence of an Acute Bout of Resistance Exercise on Circulating Endothelial Progenitor Cells and Endothelial Microparticles in Trained Men

Mark Ross

    Research output: Types of ThesisMaster's Thesis

    Abstract

    The endothelium plays an important role in maintaining vascular homeostasis. Endothelial progenitor cells (EPC) are cells which take part in endothelial repair and angiogenesis. These EPCs are typically in low circulating numbers in disease states than healthy populations. Endothelial microparticles (EMP) are blebs of endothelial cell membranes which “bud” off due to endothelial cell activation and apoptosis, and can act as a sensitive biomarker of endothelial status. We hypothesised that low intensity resistance exercise that greatly increased muscle fatigue and acidosis would increase EPCs and endothelial activation. Aim: To determine the influence of low intensity resistance exercise on EPCs and EMPs. Methods: Thirteen healthy resistance trained men undertook an acute bout of high volume low intensity resistance exercise (RE). Peripheral blood samples obtained pre- and at 10 min, 2 hr and 24 hr post-exercise. EPCs (CD34+VEGFR2+CD45dim) and EMP reflecting endothelial cell activation (CD62E+ EMP) and apoptosis (CD144+ EMP) were measured by flow cytometry. Results: Circulating EPCs were increased as a result of the RE bout. Total progenitor cell (CD34+CD45dim) counts did not change following exercise however. When EPC counts are expressed as a % of total CD45+ events, these were 74% and 110% higher (p<0.05) at 2 hr and 24 hr post-exercise respectively. CD62E+ EMP but not CD144+ EMP increased post-exercise with CD62E+ EMP 27% higher at 24 hr post-exercise. These changes in EPC and EMP were accompanied by increases in serum vascular endothelial growth factor (VEGF) and granulocyte-colony stimulating factor (G-CSF). There were no significant changes in other MP subsets. Conclusions: Low intensity resistance exercise can increase circulating EPCs to promote angiogenesis and vascular repair, possibly via increases in VEGF and G-CSF. Resistance exercise also increases the expression of the activation marker CD62E on endothelial cells which may play a role in binding circulating EPC to the endothelium.
    Original languageEnglish
    Awarding Institution
    Publication statusUnpublished - 2011

    Keywords

    • Exercise

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