Abstract
We describe the novel use of water-in-oil (W/O) microemulsions to achieve unique separations in microemulsion electrokinetic chromatography (MEEKC). The choise and concentration of the buffer type, surfactant and co-surfactant were all examined and optimized. Separations of a range of neutral and acidic analytes was shown to be markedly different to that obtained by (oil-in-water) O/W MEEKC. Neutral solutes are separated by virtue of their solubility (log P) values in O/W MEEKC with the more water-insoluble solutes migrating last. This separation process does not occur in W/O, as neutral solutes are not separated in order of log P.
| Original language | English |
|---|---|
| Pages (from-to) | 645-652 |
| Number of pages | 8 |
| Journal | Electrophoresis |
| Volume | 25 |
| Issue number | 4-5 |
| DOIs | |
| Publication status | Published - Feb 2004 |
Keywords
- Microemulsion electrokinetic chromatography
- Water-in-oil emulsion