TY - JOUR
T1 - The use of molecularly imprinted sol-gels in pharmaceutical separations
AU - Olwill, Aisling
AU - Hughes, Helen
AU - O'Riordain, Micháel
AU - McLoughlin, Peter
N1 - Funding Information:
This research was funded under the Technological Sector Research and Initiative (2001) (Strand III) supported by Merck, Sharpe & Dohme, Clonmel Ltd., Ireland. The help of Sharon Marx, Department of Physical Chemistry, Israel Institute for Biological Research, Ness-Ziona, 70450 Israel, in the initial stages of this work is also greatly appreciated.
PY - 2004/12/15
Y1 - 2004/12/15
N2 - This paper illustrates the potential of the sol-gel process to imprint the pharmaceutical active - N-[N-[(1S)-1-carboxssy-3-phenylpropyl]-l-lysyl]-l- proline, (lisinopril dihydrate). This template exhibits unique difficulties such as limited solubility in non-polar and most polar porogens with multiple functionality evident in its 4 pKa values. Selectivity for this template was achieved using a 3-monomer sol-gel system utilising solid phase extraction (SPE). Analysis of the template and its related substances was achieved using HPLC. The effect of solvent polarity on the rebinding of the template was studied. Through optimisation of porogen and extraction solvent, the imprinted material (MIP) demonstrated enhanced selectivity, for the template, over a non-imprinted material (NIP). Selectivity was also illustrated for the original template over two of its related substances. The effect of starting monomer ratio on selectivity was studied to determine the interactions, which could best be exploited to further enhance selectivity.
AB - This paper illustrates the potential of the sol-gel process to imprint the pharmaceutical active - N-[N-[(1S)-1-carboxssy-3-phenylpropyl]-l-lysyl]-l- proline, (lisinopril dihydrate). This template exhibits unique difficulties such as limited solubility in non-polar and most polar porogens with multiple functionality evident in its 4 pKa values. Selectivity for this template was achieved using a 3-monomer sol-gel system utilising solid phase extraction (SPE). Analysis of the template and its related substances was achieved using HPLC. The effect of solvent polarity on the rebinding of the template was studied. Through optimisation of porogen and extraction solvent, the imprinted material (MIP) demonstrated enhanced selectivity, for the template, over a non-imprinted material (NIP). Selectivity was also illustrated for the original template over two of its related substances. The effect of starting monomer ratio on selectivity was studied to determine the interactions, which could best be exploited to further enhance selectivity.
KW - HPLC
KW - Lisinopril
KW - Molecularly imprinted polymers
KW - Non-covalent interactions
KW - SPE
KW - Sol-gel
UR - http://www.scopus.com/inward/record.url?scp=8844280086&partnerID=8YFLogxK
U2 - 10.1016/j.bios.2004.04.029
DO - 10.1016/j.bios.2004.04.029
M3 - Article
C2 - 15556347
AN - SCOPUS:8844280086
SN - 0956-5663
VL - 20
SP - 1045
EP - 1050
JO - Biosensors and Bioelectronics
JF - Biosensors and Bioelectronics
IS - 6
ER -