Toxigenic C. difficile induced inflammatory marker expression by human intestinal epithelial cells is asymmetrical

Geraldine Canny, Denise Drudy, Padraic MacMathuna, Cliona O'Farrelly, Alan W. Baird

Research output: Contribution to journalArticlepeer-review

17 Citations (Scopus)

Abstract

Clostridium difficile infection of the intestinal epithelium and consequent pseudomembranous colitis is an important cause of morbidity and mortality. Pathogenesis has been ascribed exclusively to toxin production. Using in vitro models of human intestinal epithelial layers, we show that exposure to toxigenic C. difficile upregulates epithelial expression of IL-8 and ICAM-1, two molecules important in neutrophil chemoattraction and adhesion and subsequent inflammation. IL-8 production was also stimulated by toxin-containing supernatants. C difficile induced IL-8 release was inhibited by specific antiserum. Increased ICAM-1 expression only occurred after basolateral exposure to C. difficile while apical exposure had no effect on ICAM-1 expression. However, transepithelial electrical resistance was impaired by apical exposure to bacterial suspensions. We suggest that apical exposure to C. difficile induces changes in epithelial layer integrity which allows the bacteria and/or the toxin access to the basolateral compartment where pathogenic inflammatory mechanisms are activated.

Original languageEnglish
Pages (from-to)920-925
Number of pages6
JournalLife Sciences
Volume78
Issue number9
DOIs
Publication statusPublished - 25 Jan 2006
Externally publishedYes

Keywords

  • Clostridium difficile
  • Epithelium
  • ICAM-1
  • IL-8

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